Optical Rotatory Dispersion and Circular Dichroism

Optical Rotatory Dispersion and Circular Dichroism of

Studies of optical rotatory dispersion (ORD) and circular dichroism (CD) are reported on pepsinogen, carbamyl-, and succinylpepsinogen. From the ORD measurements in the wave length range of 600 to 300 rnp as functions of urea, temperature, and pH, it was shown that the macromolecular conformation of the three proteins differs markedly. On replacing the e-NH2 groups of the lysine residues of pep...

Circular dichroism and optical rotatory dispersion of proteins and polypeptides.

Optical rotatory dispersion, circular dichroism and far-ultraviolet spectra of avidin and streptavidin.

1. The optical-rotatory-dispersion and circular-dichroism curves of avidin showed positive Cotton effects centred at 228mmu and 280mmu, close to the ultraviolet-absorption bands of tryptophan. These effects disappeared when avidin was dissociated into sub-units in guanidine hydrochloride. 2. Binding of biotin had only a small effect on the optical-rotatory-dispersion curve of avidin. 3. The abs...

The Far Ultraviolet Optical Rotatory Dispersion, Circular Dichroism, and Absorption Spectra of a Myeloma

The optical rotatory properties of a myeloma globulin, immunoglobulin G (IgG), and a normal individual IgG in the far ultraviolet were investigated by rotatory dispersion, circular dichroism, and absorption spectroscopy. The optical rotatory dispersion of the myeloma immunoglobulin displayed two peaks, one at 205 ml* and the other at 210 rnp, and a trough at 228 mp, a lesser one at 230 mp, and ...

Conformational studies of Australia antigen by optical rotatory dispersion and circular dichroism.

The optical rotatory dispersion and circular dichroism of intact, 8 m urea- or sodium dodecyl sulfate-treated, and carbamidomethylated Australia antigen indicated that the antigen possesses a high alpha-helical content similar to human high-density lipoproteins.